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[Chal]

TRANSCRIPTIONAL REGULATION OF THE

gluB PROMOTER

DURING PLANT RESPONSE TO INFECTION

AGNIESZKA MAC, MAGDALENA KRZYMOWSKA, ANNA BARABASZ

and JACEK HENNIG*

Institute of Biochemistry and Biophysics, Polish Academy of Sciences,

Pawi

ñskiego 5A, 02-106 Warsaw, Poland

Abstract:

Several studies suggest that plant hydrolytic enzymes, such as 1,3-b-

glucanases, may be components of a general defense system against pathogen

invasion in several different plant species. We isolated and characterized

a genomic sequence coding for a new acidic 1,3-b-glucanase (gluB) from

Solanum tuberosum

. The 5' flanking region of the gluB gene was also

characterized. A chimeric gene composed of 2998 bp of the promoter sequence

from the gluB gene was fused to the b-glucuronidase (GUS) coding region and

used to transform potato and tobacco plants. Transcriptional activation of the

gluB

promoter was investigated in response to inoculation with Phytophthora

infestans (Pi) or tobacco mosaic virus (TMV). In pathogen inoculated transgenic

plants, GUS activity was strongly induced locally around necrotic lesions.

Key Words:

Plant-Pathogen Interactions, 1,3-b-glucanase, Transcriptional

Regulation



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http://www.amazon.fr/exec/obidos/ASI...524561-0717040

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